• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Beta Adducin Polyclonal antibody

Beta Adducin Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14640-1-AP

Print datasheet

Synonyms

ADD2, ADD 2, Beta-adducin, Adducin2, adducin 2 (beta)

验证数据展示

  • WB analysis using 14640-1-AP

    WB analysis using 14640-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14640-1-AP (Beta adducin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 14640-1-AP

    IP experiment of K-562 using 14640-1-AP

    IP result of anti-Beta Adducin (IP:14640-1-AP, 4ug; Detection:14640-1-AP 1:1000) with K-562 cells lysate 3200ug.

  • IHC staining of human stomach using 14640-1-AP

    IHC staining of human stomach using 14640-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14640-1-AP (Beta Adducin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14640-1-AP targets Beta Adducin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Beta Adducin fusion protein Ag6223 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adducin 2 (beta)
Synonyms ADD2, ADD 2, Beta-adducin, Adducin2, adducin 2 (beta)
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC065525
Gene Symbol Beta Adducin
Gene ID (NCBI) 119
RRIDAB_2223568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35612
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Beta Adducin antibody 14640-1-APDownload protocol
IHC protocol for Beta Adducin antibody 14640-1-APDownload protocol
IP protocol for Beta Adducin antibody 14640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Adv

Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment.

Authors - Hong Ouyang
View Article
mouseWB

Cell Rep

Pyruvate dehydrogenase complex E1 subunit α crotonylation modulates cocaine-associated memory through hippocampal neuron activation

Authors - Hongchun Li
View Article
humanWB

Science

Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells

Authors - Zhi Lin
View Article