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ADH1C Polyclonal antibody

ADH1C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18897-1-AP

Print datasheet

Synonyms

EC:1.1.1.1, ADH3

验证数据展示

  • IP experiment of mouse liver using 18897-1-AP

    IP experiment of mouse liver using 18897-1-AP

    IP result of anti-ADH1C (IP:18897-1-AP, 4ug; Detection:18897-1-AP 1:1000) with mouse liver tissue lysate 4000 ug.

  • WB analysis using 18897-1-AP

    WB analysis using 18897-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18897-1-AP (ADH1C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 18897-1-AP

    IHC staining of human lung cancer using 18897-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADH1C antibody included
  • IHC staining of human colon using 18897-1-AP

    IHC staining of human colon using 18897-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADH1C antibody included
  • IHC staining of rat liver using 18897-1-AP

    IHC staining of rat liver using 18897-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADH1C antibody included
  • IF Staining of HepG2 using 18897-1-AP

    IF Staining of HepG2 using 18897-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1C antibody (18897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inrat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inrat liver tissue, human lung cancer tissue, human colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ADH1C Ready-To-Use IHC Kit. ADH1C primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

18897-1-AP targets ADH1C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ADH1C fusion protein Ag13528 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase 1C (class I), gamma polypeptide
Synonyms EC:1.1.1.1, ADH3
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 37-45 kDa
GenBank Accession NumberBC062476
Gene Symbol ADH1C
Gene ID (NCBI) 126
RRIDAB_10596950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00326
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).

实验方案

Product Specific Protocols
WB protocol for ADH1C antibody 18897-1-APDownload protocol
IHC protocol for ADH1C antibody 18897-1-APDownload protocol
IF protocol for ADH1C antibody 18897-1-APDownload protocol
IP protocol for ADH1C antibody 18897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.

Authors - Xue Chen
View Article
human,mouseWB

Biochem Pharmacol

The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis.

Authors - Feifeng Song
View Article
mouseWB

Biochem Pharmacol

PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.

Authors - Yunhui Xu
View Article