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Species Reactivity
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验证数据展示

  • WB analysis using 30621-1-AP

    WB analysis using 30621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30621-1-AP (ADH1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ADH1C Polyclonal antibody

ADH1C Polyclonal Antibody for WB, ELISA
Cat No. 30621-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

ADH1C, Alcohol dehydrogenase 1C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, mouse colon tissue, rat colon tissue
Planning an IHC experiment? We recommend our IHCeasy ADH1C Ready-To-Use IHC Kit. ADH1C primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30621-1-AP targets ADH1C in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen ADH1C fusion protein Ag33037 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase 1C (class I), gamma polypeptide
Synonyms ADH1C, Alcohol dehydrogenase 1C
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC062476
Gene Symbol ADH1C
Gene ID (NCBI) 126
RRIDAB_3086375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00326
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alcohol dehydrogenase 1C(ADH1C), also known as ADH3, is an alcohol dehydrogenase that can catalyze ethanol oxidation to metabolite acetaldehyde. According to THE HUMAN PROTEIN ATLAS database, ADH1C locates mainly to the cytosol and plasma membrane. Genetic polymorphism of the ADH1C gene (ADH1C*1 allele) is associated with various human cancers, such as gastric cancer, oral cancer and CRC, for it encodes an alcohol dehydrogenase producing 2.5 times more acetaldehyde.(PMID: 35300114)

实验方案

Product Specific Protocols
WB protocol for ADH1C antibody 30621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols