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ADH6 Polyclonal antibody

ADH6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13288-2-AP

Print datasheet

Synonyms

EC:1.1.1.1, ADH-5

验证数据展示

  • WB analysis using 13288-2-AP

    WB analysis using 13288-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13288-2-AP (ADH6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 13288-2-AP

    IF Staining of MCF-7 using 13288-2-AP

    Immunofluorescent analysis of MCF-7 cells, using ADH6 antibody 13288-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human liver using 13288-2-AP

    IHC staining of human liver using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADH6 antibody included
  • IHC staining of mouse kidney using 13288-2-AP

    IHC staining of mouse kidney using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADH6 antibody included
  • IHC staining of rat kidney using 13288-2-AP

    IHC staining of rat kidney using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADH6 antibody included

经过测试的应用

Positive WB detected inmouse liver tissue, human liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ADH6 Ready-To-Use IHC Kit. ADH6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13288-2-AP targets ADH6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ADH6 fusion protein Ag3984 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase 6 (class V)
Synonyms EC:1.1.1.1, ADH-5
Calculated Molecular Weight 368 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC039065
Gene Symbol ADH6
Gene ID (NCBI) 130
RRIDAB_2273710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28332
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADH6 antibody 13288-2-APDownload protocol
IHC protocol for ADH6 antibody 13288-2-APDownload protocol
IF protocol for ADH6 antibody 13288-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols