ADHFE1 Polyclonal antibody
ADHFE1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue |
Positive IHC detected in | human colon tissue, human colon cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14674-1-AP targets ADHFE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | ADHFE1 fusion protein Ag6382 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | alcohol dehydrogenase, iron containing, 1 |
Synonyms | HOT, HMFT2263, Fe-containing alcohol dehydrogenase, EC:1.1.99.24, Alcohol dehydrogenase iron-containing protein 1 |
Calculated Molecular Weight | 50 kDa |
Observed Molecular Weight | 50 kDa, 40-45 kDa |
GenBank Accession Number | BC064634 |
Gene Symbol | ADHFE1 |
Gene ID (NCBI) | 137872 |
RRID | AB_2221451 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8IWW8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ADHFE1 antibody 14674-1-AP | Download protocol |
IHC protocol for ADHFE1 antibody 14674-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |