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验证数据展示

  • WB analysis using 14674-1-AP

    WB analysis using 14674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14674-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 14674-1-AP

    IHC staining of human colon using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 14674-1-AP

    IHC staining of human colon using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

ADHFE1 Polyclonal antibody

ADHFE1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14674-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HOT, HMFT2263, Fe-containing alcohol dehydrogenase, EC:1.1.99.24, Alcohol dehydrogenase iron-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14674-1-AP targets ADHFE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ADHFE1 fusion protein Ag6382 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alcohol dehydrogenase, iron containing, 1
别名 HOT, HMFT2263, Fe-containing alcohol dehydrogenase, EC:1.1.99.24, Alcohol dehydrogenase iron-containing protein 1
计算分子量 50 kDa
观测分子量 50 kDa, 40-45 kDa
GenBank蛋白编号BC064634
基因名称 ADHFE1
Gene ID (NCBI) 137872
RRIDAB_2221451
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues. It belongs to the iron-containing alcohol dehydrogenase family. ADHFE1 has 4 isoforms produced by alternative splicing with the MW of 50, 45, 32, 27 kDa.

实验方案

Product Specific Protocols
WB protocol for ADHFE1 antibody 14674-1-APDownload protocol
IHC protocol for ADHFE1 antibody 14674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Circ Cardiovasc Genet

Proteome-Based Systems Biology Analysis of the Diabetic Mouse Aorta Reveals Major Changes in Fatty Acid Biosynthesis as Potential Hallmark in Diabetes Associated Vascular Disease.

Authors - Holger Husi
View Article