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ADHFE1 Polyclonal antibody

ADHFE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14674-1-AP

Print datasheet

Synonyms

ADH8, ADHFE1, HMFT2263, HOT

验证数据展示

  • WB analysis of rat kidney using 14674-1-AP

    WB analysis of rat kidney using 14674-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 14674-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14674-1-AP

    WB analysis using 14674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14674-1-AP (ADHFE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 14674-1-AP

    IHC staining of human colon using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 14674-1-AP

    IHC staining of human colon using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inrat kidney tissue, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14674-1-AP targets ADHFE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ADHFE1 fusion protein Ag6382 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase, iron containing, 1
Synonyms ADH8, ADHFE1, HMFT2263, HOT
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa, 40-45 kDa
GenBank Accession NumberBC064634
Gene Symbol ADHFE1
Gene ID (NCBI) 137872
RRIDAB_2221451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues. It belongs to the iron-containing alcohol dehydrogenase family. ADHFE1 has 4 isoforms produced by alternative splicing with the MW of 50, 45, 32, 27 kDa.

实验方案

Product Specific Protocols
WB protocol for ADHFE1 antibody 14674-1-APDownload protocol
IHC protocol for ADHFE1 antibody 14674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Circ Cardiovasc Genet

Proteome-Based Systems Biology Analysis of the Diabetic Mouse Aorta Reveals Major Changes in Fatty Acid Biosynthesis as Potential Hallmark in Diabetes Associated Vascular Disease.

Authors - Holger Husi
View Article