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ADK Polyclonal antibody

ADK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15477-1-AP

Print datasheet

Synonyms

Adenosine 5 phosphotransferase, adenosine kinase, ADK, AK

验证数据展示

  • IHC staining of human liver cancer using 15477-1-AP

    IHC staining of human liver cancer using 15477-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15477-1-AP (ADK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 15477-1-AP

    IHC staining of human liver cancer using 15477-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15477-1-AP (ADK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15477-1-AP

    WB analysis using 15477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15477-1-AP (ADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15477-1-AP targets ADK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ADK fusion protein Ag7783 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenosine kinase
Synonyms Adenosine 5 phosphotransferase, adenosine kinase, ADK, AK
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC003568
Gene Symbol ADK
Gene ID (NCBI) 132
RRIDAB_2222085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADK antibody 15477-1-APDownload protocol
IHC protocol for ADK antibody 15477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neurochem Res

NLRP3 Inflammasome Activation Enhances ADK Expression to Accelerate Epilepsy in Mice

Authors - Haiju Zhang
View Article