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Adrenomedullin Polyclonal antibody

Adrenomedullin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10778-1-AP

Print datasheet

Synonyms

ADM, ProAM N terminal 20 peptide, Proadrenomedullin N-20 terminal peptide, Pro-adrenomedullin, PAMP

验证数据展示

  • IHC staining of human placenta using 10778-1-AP

    IHC staining of human placenta using 10778-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human kidney using 10778-1-AP

    WB analysis of human kidney using 10778-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 10778-1-AP

    IHC staining of human placenta using 10778-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 10778-1-AP

    IHC staining of mouse kidney using 10778-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 10778-1-AP

    IHC staining of mouse kidney using 10778-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human placenta using 10778-1-AP

    WB analysis of human placenta using 10778-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of fetal human brain using 10778-1-AP

    WB analysis of fetal human brain using 10778-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 10778-1-AP

    WB analysis of A549 using 10778-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 10778-1-AP

    WB analysis of Raji using 10778-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 10778-1-AP

    IF Staining of A549 using 10778-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A549 using 10778-1-AP

    IF Staining of A549 using 10778-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Adrenomedullin antibody (10778-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inhuman kidney tissue, fetal human brain tissue, Raji cells, A549 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, human pancreas cancer tissue, human kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10778-1-AP targets Adrenomedullin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Adrenomedullin fusion protein Ag1197 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adrenomedullin
Synonyms ADM, ProAM N terminal 20 peptide, Proadrenomedullin N-20 terminal peptide, Pro-adrenomedullin, PAMP
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 6 kDa
GenBank Accession NumberBC015961
Gene Symbol Adrenomedullin/ADM
Gene ID (NCBI) 133
RRIDAB_2242255
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) are two small active hormones derived from the expression of a single gene (Adm) that is expressed throughout the GI tract, including the mucosal epithelium, glandular duct cells, neuroendocrine cells, and smooth muscle cells of the GI tract, between the oral cavity and the rectum (PMID:10782362, PMID:27345325). These two peptides coexist in GI cells, where they regulate many physiological functions including vasodilation, angiogenesis, anti-inflammation, organ protection, and tissue repair. AM suppresses inflammatory cytokine production in the intestinal mucosa, improves vascular and lymphatic function, mucosal epithelial repair, and intestinal barrier function in animal models with intestinal inflammation (PMID:27965594, PMID:29311984). Molecular mass species of 18, 14, and 6 kDa were identified in tumor cell lysates and presumably represent AM precursor, processed intermediates, and the authentic peptide, respectively. There is also a 22-kDa immunoreactive species in two cancer cell lines, H720 and MCF-7 (PMID: 8798536).

实验方案

Product Specific Protocols
WB protocol for Adrenomedullin antibody 10778-1-APDownload protocol
IHC protocol for Adrenomedullin antibody 10778-1-APDownload protocol
IF protocol for Adrenomedullin antibody 10778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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