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验证数据展示

  • IHC staining of human placenta using 10778-1-AP

    IHC staining of human placenta using 10778-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human kidney using 10778-1-AP

    WB analysis of human kidney using 10778-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 10778-1-AP

    IHC staining of human placenta using 10778-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 10778-1-AP

    IHC staining of mouse kidney using 10778-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 10778-1-AP

    IHC staining of mouse kidney using 10778-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human placenta using 10778-1-AP

    WB analysis of human placenta using 10778-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of fetal human brain using 10778-1-AP

    WB analysis of fetal human brain using 10778-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 10778-1-AP

    WB analysis of A549 using 10778-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 10778-1-AP

    WB analysis of Raji using 10778-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 10778-1-AP

    IF Staining of A549 using 10778-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A549 using 10778-1-AP

    IF Staining of A549 using 10778-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Adrenomedullin antibody (10778-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Adrenomedullin Polyclonal antibody

Adrenomedullin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10778-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

ADM, ProAM N terminal 20 peptide, Proadrenomedullin N-20 terminal peptide, Pro-adrenomedullin, PAMP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue, fetal human brain tissue, Raji cells, A549 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, human pancreas cancer tissue, human kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10778-1-AP targets Adrenomedullin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 Adrenomedullin fusion protein Ag1197 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adrenomedullin
别名 ADM, ProAM N terminal 20 peptide, Proadrenomedullin N-20 terminal peptide, Pro-adrenomedullin, PAMP
计算分子量 20 kDa
观测分子量 6 kDa
GenBank蛋白编号BC015961
基因名称 Adrenomedullin/ADM
Gene ID (NCBI) 133
RRIDAB_2242255
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) are two small active hormones derived from the expression of a single gene (Adm) that is expressed throughout the GI tract, including the mucosal epithelium, glandular duct cells, neuroendocrine cells, and smooth muscle cells of the GI tract, between the oral cavity and the rectum (PMID:10782362, PMID:27345325). These two peptides coexist in GI cells, where they regulate many physiological functions including vasodilation, angiogenesis, anti-inflammation, organ protection, and tissue repair. AM suppresses inflammatory cytokine production in the intestinal mucosa, improves vascular and lymphatic function, mucosal epithelial repair, and intestinal barrier function in animal models with intestinal inflammation (PMID:27965594, PMID:29311984). Molecular mass species of 18, 14, and 6 kDa were identified in tumor cell lysates and presumably represent AM precursor, processed intermediates, and the authentic peptide, respectively. There is also a 22-kDa immunoreactive species in two cancer cell lines, H720 and MCF-7 (PMID: 8798536).

实验方案

Product Specific Protocols
WB protocol for Adrenomedullin antibody 10778-1-APDownload protocol
IHC protocol for Adrenomedullin antibody 10778-1-APDownload protocol
IF protocol for Adrenomedullin antibody 10778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

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humanWB

Prostate

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CNS Neurosci Ther

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