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ADNP Polyclonal antibody

ADNP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17987-1-AP

Print datasheet

Synonyms

KIAA0784, ADNP1, Activity-dependent neuroprotector homeobox protein, Activity-dependent neuroprotective protein

验证数据展示

  • WB analysis using 17987-1-AP

    WB analysis using 17987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17987-1-AP (ADNP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17987-1-AP

    IF Staining of HeLa using 17987-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADNP antibody (17987-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human stomach using 17987-1-AP

    IHC staining of human stomach using 17987-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17987-1-AP (ADNP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17987-1-AP targets ADNP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ADNP fusion protein Ag12414 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name activity-dependent neuroprotector homeobox
Synonyms KIAA0784, ADNP1, Activity-dependent neuroprotector homeobox protein, Activity-dependent neuroprotective protein
Calculated Molecular Weight 1102 aa, 123 kDa
Observed Molecular Weight 135-145 kDa
GenBank Accession NumberBC075794
Gene Symbol ADNP
Gene ID (NCBI) 23394
RRIDAB_2222383
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2P0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADNP antibody 17987-1-APDownload protocol
IHC protocol for ADNP antibody 17987-1-APDownload protocol
IF protocol for ADNP antibody 17987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

ADNP Upregulation Promotes Bladder Cancer Cell Proliferation via the AKT Pathway.

Authors - Shuai Zhu
View Article
mouseIHC

J Cancer

ADNP prompts the cisplatin-resistance of bladder cancer via TGF-β-mediated epithelial-mesenchymal transition (EMT) pathway.

Authors - Yu Xie
View Article
humanIF

Mol Autism

Clinical impact and in vitro characterization of ADNP variants in pediatric patients

Authors - Chuanhui Ge
View Article