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  • Featured Product
  • KD/KO Validated

ADO Polyclonal antibody

ADO Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16479-1-AP

Print datasheet

Synonyms

2 aminoethanethiol dioxygenase, ADO, C10orf22, Cysteamine dioxygenase

验证数据展示

  • WB analysis of human brain using 16479-1-AP

    WB analysis of human brain using 16479-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16479-1-AP

    IHC staining of human brain using 16479-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 16479-1-AP

    IHC staining of human brain using 16479-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, mouse spleen tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16479-1-AP targets ADO in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ADO fusion protein Ag9598 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 2-aminoethanethiol (cysteamine) dioxygenase
Synonyms 2 aminoethanethiol dioxygenase, ADO, C10orf22, Cysteamine dioxygenase
Calculated Molecular Weight 270 aa, 30 kDa
Observed Molecular Weight 28 kDa-30 kDa
GenBank Accession NumberBC018660
Gene Symbol ADO
Gene ID (NCBI) 84890
RRIDAB_2222204
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SZ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADO antibody 16479-1-APDownload protocol
IHC protocol for ADO antibody 16479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Discov

ADO/hypotaurine: a novel metabolic pathway contributing to glioblastoma development.

Authors - Dachuan Shen
  • KO Validated
View Article