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ADPRH Polyclonal antibody

ADPRH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17911-1-AP

Print datasheet

Synonyms

ADP ribosylarginine hydrolase, ADPRH, ARH1

验证数据展示

  • WB analysis using 17911-1-AP

    WB analysis using 17911-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17911-1-AP (ADPRH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17911-1-AP

    IHC staining of mouse brain using 17911-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17911-1-AP (ADPRH antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17911-1-AP

    IHC staining of mouse brain using 17911-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17911-1-AP (ADPRH antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17911-1-AP targets ADPRH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ADPRH fusion protein Ag12311 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylarginine hydrolase
Synonyms ADP ribosylarginine hydrolase, ADPRH, ARH1
Calculated Molecular Weight 357 aa, 40 kDa
Observed Molecular Weight 36-39 kDa
GenBank Accession NumberBC063883
Gene Symbol ADPRH
Gene ID (NCBI) 141
RRIDAB_2878466
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54922
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADPRH antibody 17911-1-APDownload protocol
IHC protocol for ADPRH antibody 17911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Cancer

ADPRH is a prognosis-related biomarker and correlates with immune infiltrates in low grade glioma.

Authors - Chunyu Zhang
View Article