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ARH3 Polyclonal antibody

ARH3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16504-1-AP

Print datasheet

Synonyms

ADP-ribosylhydrolase 3, ADP-ribose glycohydrolase ARH3, ADPRHL2, ADPRHL 2, [Protein ADP-ribosylserine] hydrolase

验证数据展示

  • WB analysis using 16504-1-AP

    WB analysis using 16504-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 16504-1-AP

    IHC staining of mouse testis using 16504-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 16504-1-AP

    IHC staining of mouse testis using 16504-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16504-1-AP

    IF Staining of HepG2 using 16504-1-AP

    Immunofluorescent analysis of HepG2 cells using 16504-1-AP (ARH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, HepG2 cells, mouse heart tissue, mouse lung tissue, mouse kidney tissue, mouse thymus tissue, rat brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16504-1-AP targets ARH3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen ARH3 fusion protein Ag9683 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylhydrolase like 2
Synonyms ADP-ribosylhydrolase 3, ADP-ribose glycohydrolase ARH3, ADPRHL2, ADPRHL 2, [Protein ADP-ribosylserine] hydrolase
Calculated Molecular Weight 363 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC014169
Gene Symbol ARH3
Gene ID (NCBI) 54936
RRIDAB_2224919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX46
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARH3 can be detected as two bands between 39 and 40 kDa.(PMID: 16278211/32636369)

实验方案

Product Specific Protocols
WB protocol for ARH3 antibody 16504-1-APDownload protocol
IHC protocol for ARH3 antibody 16504-1-APDownload protocol
IF protocol for ARH3 antibody 16504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,ratWB,IP

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
View Article