验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, HeLa cells, mouse brain tissue |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19777-1-AP targets ADRA1A-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adrenergic, alpha-1A-, receptor |
| 别名 | ADRA1A, Alpha-1C adrenergic receptor, Alpha-1A adrenoreceptor, Alpha-1A adrenoceptor, Alpha-1A adrenergic receptor |
| 计算分子量 | 51 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | NM_000680 |
| 基因名称 | ADRA1A |
| Gene ID (NCBI) | 148 |
| RRID | AB_10643238 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35348 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADRA1A, also named as ADRA1C, belongs to the G-protein coupled receptor 1 family. ADRA1A mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. The antibody is specific to ADRA1A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADRA1A-Specific antibody 19777-1-AP | Download protocol |
| FC protocol for ADRA1A-Specific antibody 19777-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Immunol Chronic stimulation desensitizes β2-adrenergic receptor responses in natural killer cells | ||
iScience Changes in transcriptomic landscape in human end-stage heart failure with distinct etiology. | ||
Pharmaceuticals (Basel) Vascular α1A Adrenergic Receptors as a Potential Therapeutic Target for IPAD in Alzheimer's Disease. | ||
Vascul Pharmacol The ERK1/2 pathway participates in the upregulation of the expression of mesenteric artery α1 receptors by intravenous tail injections of mmLDL in mice. | ||
Heliyon Luteolin improves vasoconstriction function and survival of septic mice via AMPK/NF-κB pathway | ||
BMC Neurosci Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α2A-adrenergic receptor |
