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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of PC-3 using 22419-1-AP

    FC experiment of PC-3 using 22419-1-AP

    1x10^6 PC-3 cells were intracellularly stained with 0.8 ug ADRA1B Polyclonal antibody (22419-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 22419-1-AP

    WB analysis using 22419-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22419-1-AP (ADRA1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ADRA1B Polyclonal antibody

ADRA1B Polyclonal Antibody for WB, ELISA
Cat No. 22419-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA and More (1)

别名

Alpha-1B adrenoceptor, Alpha-1B adrenergic receptor, Alpha 1B adrenoreceptor, Alpha 1B adrenoceptor, Alpha 1B adrenergic receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse testis tissue, rat brain tissue
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22419-1-AP targets ADRA1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 ADRA1B fusion protein Ag18138 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adrenergic, alpha-1B-, receptor
别名 Alpha-1B adrenoceptor, Alpha-1B adrenergic receptor, Alpha 1B adrenoreceptor, Alpha 1B adrenoceptor, Alpha 1B adrenergic receptor
计算分子量 520 aa, 57 kDa
观测分子量 70 kDa
GenBank蛋白编号BC136569
基因名称 ADRA1B
Gene ID (NCBI) 147
RRIDAB_2879103
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP35368
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADRA1B antibody 22419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC,WB

Chin Med

Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.

Authors - Xian Zhang
View Article
ratWB

Eur J Pharmacol

Alpha1B-adreneroceptor is involved in norepinephrine-induced pulmonary artery smooth muscle cell proliferation via p38 signaling

Authors - Xiaolin Xiao
View Article
humanWB

Mol Med Rep

Stigmasterol exerts antiglioma effects by regulating lipid metabolism

Authors - Ting Wei
View Article
humanIHC

Onco Targets Ther

Sympathetic innervation contributes to perineural invasion of salivary adenoid cystic carcinoma via the β2-adrenergic receptor.

Authors - Chao Ma
View Article