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ADRA1B Polyclonal antibody

ADRA1B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22419-1-AP

Print datasheet

Synonyms

Alpha-1B adrenoceptor, Alpha-1B adrenergic receptor, Alpha 1B adrenoreceptor, Alpha 1B adrenoceptor, Alpha 1B adrenergic receptor

验证数据展示

  • FC experiment of PC-3 using 22419-1-AP

    FC experiment of PC-3 using 22419-1-AP

    1x10^6 PC-3 cells were intracellularly stained with 0.8 ug ADRA1B Polyclonal antibody (22419-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 22419-1-AP

    WB analysis using 22419-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22419-1-AP (ADRA1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse testis tissue, rat brain tissue
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22419-1-AP targets ADRA1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen ADRA1B fusion protein Ag18138 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adrenergic, alpha-1B-, receptor
Synonyms Alpha-1B adrenoceptor, Alpha-1B adrenergic receptor, Alpha 1B adrenoreceptor, Alpha 1B adrenoceptor, Alpha 1B adrenergic receptor
Calculated Molecular Weight 520 aa, 57 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC136569
Gene Symbol ADRA1B
Gene ID (NCBI) 147
RRIDAB_2879103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP35368
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADRA1B antibody 22419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC,WB

Chin Med

Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.

Authors - Xian Zhang
View Article
ratWB

Eur J Pharmacol

Alpha1B-adreneroceptor is involved in norepinephrine-induced pulmonary artery smooth muscle cell proliferation via p38 signaling

Authors - Xiaolin Xiao
View Article
humanIHC

Onco Targets Ther

Sympathetic innervation contributes to perineural invasion of salivary adenoid cystic carcinoma via the β2-adrenergic receptor.

Authors - Chao Ma
View Article