验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30713-1-AP targets ADRB3 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | ADRB3 fusion protein Ag31466 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adrenergic, beta-3-, receptor |
| 别名 | ADRB3, ADRB3R, adrenergic, beta 3 , receptor, B3AR, Beta 3 adrenergic receptor, Beta 3 adrenoceptor, Beta 3 adrenoreceptor, BETA3AR, FLJ99960 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44-50 kDa |
| GenBank蛋白编号 | NM_000025 |
| 基因名称 | ADRB3 |
| Gene ID (NCBI) | 155 |
| RRID | AB_3086396 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13945 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-3 adrenergic receptor(ADRB3), a member of the G-protein-coupled receptor family, is expressed mainly in adipose tissue and is thought to contribute to lipolysis and thermogenesis. In addition, the overexpression of ADRB3 has been found in several cancer types, including breast cancer, gallbladder cancer , and colorectal cancer. More recently, the involvement of ADRB3 in the metabolic reprogramming of melanoma, the promotion of immune tolerance, and the regulation of cancer differentiation have been described. (PMID: 32514619)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADRB3 antibody 30713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |