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GRK3 Polyclonal antibody

GRK3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13727-1-AP

Print datasheet

Synonyms

G protein-coupled receptor kinase 3, EC:2.7.11.15, Beta-ARK-2, Beta-adrenergic receptor kinase 2, ADRBK2

验证数据展示

  • WB analysis of K-562 using 13727-1-AP

    WB analysis of K-562 using 13727-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13727-1-AP (GRK3 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13727-1-AP

    IHC staining of mouse brain using 13727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13727-1-AP (GRK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13727-1-AP

    IHC staining of mouse brain using 13727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13727-1-AP (GRK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 13727-1-AP

    IHC staining of human colon using 13727-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13727-1-AP (GRK3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, HL-60 cells
Positive IHC detected inmouse brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13727-1-AP targets GRK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GRK3 fusion protein Ag4685 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adrenergic, beta, receptor kinase 2
Synonyms G protein-coupled receptor kinase 3, EC:2.7.11.15, Beta-ARK-2, Beta-adrenergic receptor kinase 2, ADRBK2
Calculated Molecular Weight 688 aa, 80 kDa
Observed Molecular Weight 70 kDa, 90 kDa
GenBank Accession NumberBC036797
Gene Symbol GRK3
Gene ID (NCBI) 157
RRIDAB_2225851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRK3 (G protein coupled receptor kinase 3), also known as ADRBK2 or BARK2, belongs to the G protein coupled receptor kinases (GRKs) family. GRKs are serine/threonine kinases that regulate a large and diverse class of G-protein coupled receptors (GPCRs). GRKs have been shown to play a critical role in the outcome of a variety of physiological and pathophysiological processes including chemotaxis, signaling, migration, inflammatory gene expression and so on (PMID: 28950947, 29483837). GRK3 was identified as a negative regulator of CXCL12/CXCR4 signaling, and has also been shown to be overexpressed in human prostate cancer and found to promote tumor growth and metastasis by enhancing angiogenesis (PMID: 23935208, 24434559). GRK3 has a molecular mass of 75-80 kDa, and also can be ubiquitinated (PMID: 20596523).

实验方案

Product Specific Protocols
WB protocol for GRK3 antibody 13727-1-APDownload protocol
IHC protocol for GRK3 antibody 13727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols