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验证数据展示

  • WB analysis of K-562 using 13727-1-AP

    WB analysis of K-562 using 13727-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13727-1-AP (GRK3 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13727-1-AP

    IHC staining of mouse brain using 13727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13727-1-AP (GRK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13727-1-AP

    IHC staining of mouse brain using 13727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13727-1-AP (GRK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 13727-1-AP

    IHC staining of human colon using 13727-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13727-1-AP (GRK3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GRK3 Polyclonal antibody

GRK3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13727-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

G protein-coupled receptor kinase 3, EC:2.7.11.15, Beta-ARK-2, Beta-adrenergic receptor kinase 2, ADRBK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HL-60 cells
Positive IHC detected inmouse brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13727-1-AP targets GRK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GRK3 fusion protein Ag4685 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adrenergic, beta, receptor kinase 2
别名 G protein-coupled receptor kinase 3, EC:2.7.11.15, Beta-ARK-2, Beta-adrenergic receptor kinase 2, ADRBK2
计算分子量 688 aa, 80 kDa
观测分子量 70 kDa, 90 kDa
GenBank蛋白编号BC036797
基因名称 GRK3
Gene ID (NCBI) 157
RRIDAB_2225851
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35626
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRK3 (G protein coupled receptor kinase 3), also known as ADRBK2 or BARK2, belongs to the G protein coupled receptor kinases (GRKs) family. GRKs are serine/threonine kinases that regulate a large and diverse class of G-protein coupled receptors (GPCRs). GRKs have been shown to play a critical role in the outcome of a variety of physiological and pathophysiological processes including chemotaxis, signaling, migration, inflammatory gene expression and so on (PMID: 28950947, 29483837). GRK3 was identified as a negative regulator of CXCL12/CXCR4 signaling, and has also been shown to be overexpressed in human prostate cancer and found to promote tumor growth and metastasis by enhancing angiogenesis (PMID: 23935208, 24434559). GRK3 has a molecular mass of 75-80 kDa, and also can be ubiquitinated (PMID: 20596523).

实验方案

Product Specific Protocols
WB protocol for GRK3 antibody 13727-1-APDownload protocol
IHC protocol for GRK3 antibody 13727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols