验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, K-562 cells, PC-3 cells, Raji cells, NIH/3T3 cells |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11468-1-AP targets ADRM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | ADRM1 fusion protein Ag1997 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | adhesion regulating molecule 1 |
别名 | GP110, ARM-1, ARM1, ARM 1, ADRM 1 |
计算分子量 | 407 aa, 42 kDa |
观测分子量 | 46 kDa |
GenBank蛋白编号 | BC017245 |
基因名称 | ADRM1 |
Gene ID (NCBI) | 11047 |
RRID | AB_2273873 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q16186 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.
实验方案
Product Specific Protocols | |
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WB protocol for ADRM1 antibody 11468-1-AP | Download protocol |
IHC protocol for ADRM1 antibody 11468-1-AP | Download protocol |
IF protocol for ADRM1 antibody 11468-1-AP | Download protocol |
IP protocol for ADRM1 antibody 11468-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Exp Hematol Oncol Downregulation of pro-surfactant protein B contributes to the recurrence of early-stage non-small cell lung cancer by activating PGK1-mediated Akt signaling
| ||
Proc Natl Acad Sci U S A Dendritic spinopathy in transgenic mice expressing ALS/dementia-linked mutant UBQLN2. | ||
Oncogene The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a. | ||
Genes Chromosomes Cancer Comprehensive analysis of 20q13 genes in ovarian cancer identifies ADRM1 as amplification target. |