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验证数据展示

  • WB analysis using 26332-1-AP

    WB analysis using 26332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26332-1-AP (AE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 26332-1-AP

    IHC staining of human liver cancer using 26332-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 26332-1-AP

    IHC staining of human liver cancer using 26332-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AE2 Polyclonal antibody

AE2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26332-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

AE 2, AE2, Anion exchange protein 2, Anion exchanger 2, BND3L, EPB3L1, HKB3, MPB3L, NBND3, SLC4A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26332-1-AP targets AE2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 AE2 fusion protein Ag23673 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 4, anion exchanger, member 2 (erythrocyte membrane protein band 3-like 1)
别名 AE 2, AE2, Anion exchange protein 2, Anion exchanger 2, BND3L, EPB3L1, HKB3, MPB3L, NBND3, SLC4A2
计算分子量 1241 aa, 137 kDa
观测分子量 150 and 70 kDa
GenBank蛋白编号BC028601
基因名称 AE2
Gene ID (NCBI) 6522
RRIDAB_2880481
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04920
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC4A2, also named as AE2, encodes a Cl-/HCO3- exchanger. It has been reported that the reduced expression of SLC4A2 found in the liver and peripheral blood mononuclear cells of patients may be associated with the hypermethylation of SLC4A2 promoter regions, which might contribute to the pathogenesis of primary biliary cholangitis (PBC). 26332-1-AP antibody detects 150 kDa and 70 kDa (XP_006716157, 666aa) protein in SDS-PAGE. (PMID: 23341620, 32039364)

实验方案

Product Specific Protocols
WB protocol for AE2 antibody 26332-1-APDownload protocol
IHC protocol for AE2 antibody 26332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.

Authors - Eunus S Ali
View Article