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验证数据展示

  • WB analysis using 68592-1-Ig (same clone as 68592-1-PBS)

    WB analysis using 68592-1-Ig (same clone as 68592-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68592-1-Ig (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68592-1-Ig (same clone as 68592-1-PBS)

    IF Staining of A431 using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using AEG-1/MTDH antibody (68592-1-Ig, Clone: 1D8C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

  • IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

    IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTDH antibody included
  • IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

    IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTDH antibody included
  • IHC staining of human tonsillitis using 68592-1-Ig (same clone as 68592-1-PBS)

    IHC staining of human tonsillitis using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTDH antibody included
  • IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

    IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTDH antibody included

AEG-1/MTDH Monoclonal antibody, PBS Only

AEG-1/MTDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68592-1-PBS

产品说明书

CloneNo. 1D8C2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AEG-1, MTDH, 1D8C2, 3D3/LYRIC, AEG 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MTDH Ready-To-Use IHC Kit. MTDH primary antibody included.

产品信息

68592-1-PBS targets AEG-1/MTDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 AEG-1/MTDH fusion protein Ag5033 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 metadherin
别名 AEG-1, MTDH, 1D8C2, 3D3/LYRIC, AEG 1
计算分子量 582 aa, 64 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC045642
基因名称 AEG-1/MTDH
Gene ID (NCBI) 92140
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86UE4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.