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验证数据展示

  • WB analysis using 23913-1-AP

    WB analysis using 23913-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23913-1-AP (AES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

AES Polyclonal antibody

AES Polyclonal Antibody for WB, ELISA
Cat No. 23913-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

AES, AES 1, AES 2, Amino enhancer of split, ESP1, Gp130 associated protein GAM, GRG, GRG5, Protein ESP1, Protein GRG, TLE5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23913-1-AP targets AES in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AES fusion protein Ag21021 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 amino-terminal enhancer of split
别名 AES, AES 1, AES 2, Amino enhancer of split, ESP1, Gp130 associated protein GAM, GRG, GRG5, Protein ESP1, Protein GRG, TLE5
计算分子量 264 aa, 29 kDa
观测分子量 22-29 kDa
GenBank蛋白编号BC113735
基因名称 AES
Gene ID (NCBI) 166
RRIDAB_3669425
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ08117
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069).

实验方案

Product Specific Protocols
WB protocol for AES antibody 23913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols