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AFG3L2 Polyclonal antibody
AFG3L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse skeletal muscle tissue, mouse kidney tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat liver tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14631-1-AP targets AFG3L2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, zebrafish |
Immunogen | AFG3L2 fusion protein Ag6209 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | AFG3 ATPase family gene 3-like 2 (yeast) |
Synonyms | Paraplegin-like protein, Mitochondrial inner membrane m-AAA protease component AFG3L2, EC:3.4.24.-, AFG3-like protein 2, AFG3L 2 |
Calculated Molecular Weight | 88 kDa |
Observed Molecular Weight | 80-90 kDa |
GenBank Accession Number | BC065016 |
Gene Symbol | AFG3L2 |
Gene ID (NCBI) | 10939 |
RRID | AB_2242420 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y4W6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for AFG3L2 antibody 14631-1-AP | Download protocol |
IHC protocol for AFG3L2 antibody 14631-1-AP | Download protocol |
IF protocol for AFG3L2 antibody 14631-1-AP | Download protocol |
IP protocol for AFG3L2 antibody 14631-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex
| ||
Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. | ||
Cancer Res Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition | ||
Brain Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance. |