AFG3L2 Monoclonal antibody
AFG3L2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1C12F3
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, pig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue |
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68517-1-Ig targets AFG3L2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat, pig, rabbit |
Immunogen | AFG3L2 fusion protein Ag7180 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | AFG3 ATPase family gene 3-like 2 (yeast) |
Synonyms | Mitochondrial inner membrane m-AAA protease component AFG3L2, EC:3.4.24.-, AFG3-like protein 2, AFG3L 2, AFG3 like protein 2 |
Calculated Molecular Weight | 88 kDa |
Observed Molecular Weight | 80 kDa |
GenBank Accession Number | BC065016 |
Gene Symbol | AFG3L2 |
Gene ID (NCBI) | 10939 |
RRID | AB_3085226 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9Y4W6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for AFG3L2 antibody 68517-1-Ig | Download protocol |
IF protocol for AFG3L2 antibody 68517-1-Ig | Download protocol |
FC protocol for AFG3L2 antibody 68517-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |