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  • 概述
  • 经过测试的应用
  • 产品信息
  • 实验方案

AFG3L2 Monoclonal antibody

AFG3L2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C12F3

Cat no : 68517-1-Ig

Print datasheet

Synonyms

Mitochondrial inner membrane m-AAA protease component AFG3L2, EC:3.4.24.-, AFG3-like protein 2, AFG3L 2, AFG3 like protein 2

验证数据展示

  • WB analysis using 68517-1-Ig

    WB analysis using 68517-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68517-1-Ig (AFG3L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 68517-1-Ig

    IF Staining of MCF-7 using 68517-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AFG3L2 antibody (68517-1-Ig, Clone: 1C12F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

  • FC experiment of HeLa using 68517-1-Ig

    FC experiment of HeLa using 68517-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AFG3L2 Monoclonal antibody (68517-1-Ig, Clone:1C12F3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, pig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68517-1-Ig targets AFG3L2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen AFG3L2 fusion protein Ag7180 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name AFG3 ATPase family gene 3-like 2 (yeast)
Synonyms Mitochondrial inner membrane m-AAA protease component AFG3L2, EC:3.4.24.-, AFG3-like protein 2, AFG3L 2, AFG3 like protein 2
Calculated Molecular Weight 88 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC065016
Gene Symbol AFG3L2
Gene ID (NCBI) 10939
RRIDAB_3085226
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4W6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).

实验方案

Product Specific Protocols
WB protocol for AFG3L2 antibody 68517-1-IgDownload protocol
IF protocol for AFG3L2 antibody 68517-1-IgDownload protocol
FC protocol for AFG3L2 antibody 68517-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols