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AFM Polyclonal antibody

AFM Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19494-1-AP

Print datasheet

Synonyms

Alpha Alb, ALF, ALBA, ALB2, afamin/AFM

验证数据展示

  • WB analysis of human plasma using 19494-1-AP

    WB analysis of human plasma using 19494-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 19494-1-AP (AFM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 19494-1-AP

    IF Staining of HeLa using 19494-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (19494-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inhuman blood tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19494-1-AP targets AFM in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name afamin
Synonyms Alpha Alb, ALF, ALBA, ALB2, afamin/AFM
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberNM_001133
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2878581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

实验方案

Product Specific Protocols
WB protocol for AFM antibody 19494-1-APDownload protocol
IF protocol for AFM antibody 19494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Afamin promotes glucose metabolism in papillary thyroid carcinoma.

Authors - Chen-Tian Shen
View Article