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AFM Polyclonal antibody

AFM Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25280-1-AP

Print datasheet

Synonyms

afamin, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin

验证数据展示

  • WB analysis of human blood using 25280-1-AP

    WB analysis of human blood using 25280-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 25280-1-AP (AFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of human plasma using 25280-1-AP

    IP experiment of human plasma using 25280-1-AP

    IP result of anti-AFM (IP:25280-1-AP, 4ug; Detection:25280-1-AP 1:2000) with human plasma lysate 4000ug.

经过测试的应用

Positive WB detected inhuman blood tissue
Positive IP detected inhuman plasma tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25280-1-AP is AFM Fusion Protein expressed in E. coli.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen AFM fusion protein Ag16866 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name afamin
Synonyms afamin, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin
Calculated Molecular Weight 599 aa, 69 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC109020
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2880005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

实验方案

Product Specific Protocols
WB protocol for AFM antibody 25280-1-APDownload protocol
IP protocol for AFM antibody 25280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols