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AFMID Polyclonal antibody

AFMID Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19522-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

AFMID, arylformamidase, KF, Kynurenine formamidase, Probable arylformamidase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver tissue, human liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19522-1-AP targets AFMID in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 arylformamidase
别名 AFMID, arylformamidase, KF, Kynurenine formamidase, Probable arylformamidase
计算分子量 35 kDa
观测分子量 30 kDa
GenBank蛋白编号NM_001145526
基因名称 AFMID
Gene ID (NCBI) 125061
RRIDAB_11041712
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ63HM1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFMID,also named as KF, belongs to the AFMID family. AFMID catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H). AFMID is required for elimination of toxic metabolites.

实验方案

Product Specific Protocols
WB protocol for AFMID antibody 19522-1-APDownload protocol
IHC protocol for AFMID antibody 19522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, mouseWB

Cancer Cell

Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.

Authors - Krishna S Tummala
  • KD Validated
humanWB

Genome Res

A human-specific switch of alternatively spliced AFMID isoforms contributes to TP53 mutations and tumor recurrence in hepatocellular carcinoma.

Authors - Kuan-Ting Lin
mouseWB

Mol Cell Endocrinol

Hepatic NAD+levels and NAMPT abundance are unaffected during prolonged high-fat diet consumption in C57BL/6JBomTac mice.

Authors - Morten Dall
humanWB

J Proteome Res

Proteomics Analysis of Renal Cell Line Caki-2 with AFMID Overexpression and Potential Biomarker Discovery in Urine

Authors - Jiameng Sun
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