AFP Polyclonal antibody

AFP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 14550-1-AP

Print datasheet

Synonyms

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP



经过测试的应用

Positive WB detected inBxPC-3 cells, HuH-7 cells, human placenta tissue, L02 cells, HepG2cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14550-1-AP targets AFP in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications CoIP, FC, IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen AFP fusion protein Ag6089 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alpha-fetoprotein
Synonyms AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC027881
Gene Symbol AFP
Gene ID (NCBI) 174
RRIDAB_2223933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).

实验方案

Product Specific Protocols
WB protocol for AFP antibody 14550-1-APDownload protocol
IHC protocol for AFP antibody 14550-1-APDownload protocol
IF protocol for AFP antibody 14550-1-APDownload protocol
IP protocol for AFP antibody 14550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Cell

Glycogen accumulation and phase separation drives liver tumor initiation.

Authors - Qingxu Liu
humanIHC

Cancer Cell

Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes.

Authors - Ruidong Xue
mouseIHC

J Clin Invest

Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.

Authors - Xueru Mu
humanIHC

J Clin Invest

HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis.

Authors - Celine Hernandez
human,mouseWB,IHC,IF

Nat Commun

Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma.

Authors - Wei Cheng
humanIHC

Nat Commun

Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.

Authors - Jun Lu