AFP Polyclonal antibody
AFP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
109
验证数据展示
经过测试的应用
Positive WB detected in | BxPC-3 cells, HuH-7 cells, human placenta tissue, L02 cells, HepG2cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14550-1-AP targets AFP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, goat |
Immunogen | AFP fusion protein Ag6089 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | alpha-fetoprotein |
Synonyms | HPAFP, Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein |
Calculated Molecular Weight | 69 kDa |
Observed Molecular Weight | 68-72 kDa |
GenBank Accession Number | BC027881 |
Gene Symbol | AFP |
Gene ID (NCBI) | 174 |
RRID | AB_2223933 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P02771 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for AFP antibody 14550-1-AP | Download protocol |
IHC protocol for AFP antibody 14550-1-AP | Download protocol |
IF protocol for AFP antibody 14550-1-AP | Download protocol |
IP protocol for AFP antibody 14550-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Cell Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes. | ||
J Clin Invest Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment. | ||
J Clin Invest HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis. | ||
Nat Commun Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma. | ||
Nat Commun Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets. |