• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HepG2 using 67852-1-Ig (same clone as 67852-1-PBS)

    WB analysis of HepG2 using 67852-1-Ig (same clone as 67852-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • WB analysis of HuH-7 using 67852-1-Ig (same clone as 67852-1-PBS)

    WB analysis of HuH-7 using 67852-1-Ig (same clone as 67852-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 67852-1-Ig (same clone as 67852-1-PBS)

    WB analysis of human testis using 67852-1-Ig (same clone as 67852-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 67852-1-Ig (same clone as 67852-1-PBS)

    WB analysis of human placenta using 67852-1-Ig (same clone as 67852-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

    IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

    IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

    IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

    IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included

AFP Monoclonal antibody, PBS Only

AFP Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67852-1-PBS

产品说明书

CloneNo. 4B3E4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

产品信息

67852-1-PBS targets AFP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 AFP fusion protein Ag6089 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alpha-fetoprotein
别名 Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin
计算分子量 69 kDa
观测分子量 68-72 kDa
GenBank蛋白编号BC027881
基因名称 AFP
Gene ID (NCBI) 174
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02771
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)