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验证数据展示

  • Sandwich ELISA standard curve of MP00001-4

    Sandwich ELISA standard curve of MP00001-4

    Sandwich ELISA standard curve of MP00001-4, AFP Recombinant Matched Antibody Pair - PBS only. 82982-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0539. 82982-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00001-4

    Sample test of MP00001-4

    HepG2 (human hepatocellular carcinoma cells) were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean AFP concentration was determined to be 3,038.3 ng/mL in HepG2 supernatant.

  • Sample test of MP00001-4

    Sample test of MP00001-4

    Serum of sixteen individual healthy human donors was measured. The AFP concentration of detected samples was determined to be 8,740.7 pg/mL with a range of 2,872.1 - 32,900 pg/mL.

  • Cytometric bead array standard curve of MP00001-2

    Cytometric bead array standard curve of MP00001-2

    Cytometric bead array standard curve of MP00001-2, AFP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82982-3-PBS. Detection antibody: 82982-2-PBS. Standard: Eg0539. Range: 1.25-20 ng/mL.

AFP Recombinant antibody, PBS Only (Capture)

AFP Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 82982-3-PBS

产品说明书

CloneNo. 230435G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

产品信息

82982-3-PBS targets AFP as part of a matched antibody pair:

MP00001-2: 82982-3-PBS capture and 82982-2-PBS detection (validated in Cytometric bead array)

MP00001-4: 82982-3-PBS capture and 82982-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alpha-fetoprotein
别名 Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin
计算分子量 69 kDa
GenBank蛋白编号BC027881
基因名称 AFP
Gene ID (NCBI) 174
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02771
储存缓冲液 100% PBS pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C