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AFP Recombinant antibody, PBS Only (Capture/Detector)

AFP Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230435A11

Cat no : 82982-4-PBS

Print datasheet

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

验证数据展示

  • Sandwich ELISA standard curve of MP00001-4

    Sandwich ELISA standard curve of MP00001-4

    Sandwich ELISA standard curve of MP00001-4, AFP Recombinant Matched Antibody Pair - PBS only. 82982-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0539. 82982-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00001-4

    Sample test of MP00001-4

    HepG2 (human hepatocellular carcinoma cells) were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean AFP concentration was determined to be 3,038.3 ng/mL in HepG2 supernatant.

  • Sample test of MP00001-4

    Sample test of MP00001-4

    Serum of sixteen individual healthy human donors was measured. The AFP concentration of detected samples was determined to be 8,740.7 pg/mL with a range of 2,872.1 - 32,900 pg/mL.

  • Cytometric bead array standard curve of MP00001-3

    Cytometric bead array standard curve of MP00001-3

    Cytometric bead array standard curve of MP00001-3, AFP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82982-4-PBS. Detection antibody: 82982-2-PBS. Standard: Eg0539. Range: 0.625-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

产品信息

82982-4-PBS targets AFP as part of a matched antibody pair:

MP00001-3: 82982-4-PBS capture and 82982-2-PBS detection (validated in Cytometric bead array)

MP00001-4: 82982-3-PBS capture and 82982-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alpha-fetoprotein
Synonyms Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin
Calculated Molecular Weight 69 kDa
GenBank Accession NumberBC027881
Gene Symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02771
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C