AFP Recombinant antibody

AFP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230435A2

Cat no : 82982-5-RR

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Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin



经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, human placenta
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82982-5-RR targets AFP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alpha-fetoprotein
Synonyms Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin
Calculated Molecular Weight 69 kDa
Observed Molecular Weight68-72 kDa
GenBank Accession NumberBC027881
Gene Symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)

实验方案

Product Specific Protocols
WB protocol for AFP antibody 82982-5-RRDownload protocol
IF protocol for AFP antibody 82982-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols