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AFP Recombinant antibody, PBS Only

AFP Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230435E3

Cat no : 82982-6-PBS

Print datasheet

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

验证数据展示

  • WB analysis of human placenta using 82982-6-RR (same clone as 82982-6-PBS)

    WB analysis of human placenta using 82982-6-RR (same clone as 82982-6-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 82982-6-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82982-6-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 82982-6-RR (same clone as 82982-6-PBS)

    IHC staining of human liver using 82982-6-RR (same clone as 82982-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82982-6-RR (AFP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82982-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human liver using 82982-6-RR (same clone as 82982-6-PBS)

    IHC staining of human liver using 82982-6-RR (same clone as 82982-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82982-6-RR (AFP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82982-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • Affinity and Kinetic Characterization of 82982-6-RR

    Affinity and Kinetic Characterization of 82982-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82982-6-RR against Human AFP were performed. The affinity constant is 2.80 nM.

Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

产品信息

82982-6-PBS targets AFP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alpha-fetoprotein
Synonyms Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin
Calculated Molecular Weight 69 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC027881
Gene Symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02771
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)