AGA Recombinant antibody

AGA Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83442-6-RR

产品说明书

CloneNo. 240396B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

Glycosylasparaginase alpha chain, GA, EC:3.5.1.26, ASRG, AGU

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells, mouse testis tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

83442-6-RR targets AGA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 AGA fusion protein Ag10681 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aspartylglucosaminidase
别名 Glycosylasparaginase alpha chain, GA, EC:3.5.1.26, ASRG, AGU
计算分子量 346 aa, 37 kDa
观测分子量24 kDa
GenBank蛋白编号BC012392
基因名称 AGA
Gene ID (NCBI) 175
RRIDAB_3671083
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP20933
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGA(Aspartylglucosaminidase) is also named as N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase and glycosylasparaginase.The open reading frame of the readthrough transcript would indicate AGA close to 40 kDa. It can be cleaved into 2 chains:the 24 kDa α- and 17 kDa β-subunits of the wild-type AGA,in addition,an aberrant polypeptide with a mol.wt of 21 kDa is also detected as a product of proteolytic cleavage of the mutant precursor molecule(PMID: 9425233).AGA can exsit as a dimer with the mol.wt between 100 kDa and 49 kDa and His124 at an interface between two heterodimers of AGA is crucial for the thermodynamically stable oligomeric structure of AGA.(PMID:9737998). It can also exsit as a heterotetrameric protein of 88 kDa(PMID:1733831).

实验方案

Product Specific Protocols
WB protocol for AGA antibody 83442-6-RRDownload protocol
IHC protocol for AGA antibody 83442-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Identification and experimental validation of hub genes underlying depressive-like behaviors induced by chronic social defeat stress

Authors - Yexiang Chen
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