AGA Recombinant antibody
AGA Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240396B4
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HepG2 cells, mouse testis tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83442-6-RR is AGA Fusion Protein expressed in E. coli.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, Mouse |
Immunogen | AGA fusion protein Ag10681 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | aspartylglucosaminidase |
Synonyms | Glycosylasparaginase alpha chain, GA, EC:3.5.1.26, ASRG, AGU |
Calculated Molecular Weight | 346 aa, 37 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | BC012392 |
Gene Symbol | AGA |
Gene ID (NCBI) | 175 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P20933 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AGA(Aspartylglucosaminidase) is also named as N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase and glycosylasparaginase.The open reading frame of the readthrough transcript would indicate AGA close to 40 kDa. It can be cleaved into 2 chains:the 24 kDa α- and 17 kDa β-subunits of the wild-type AGA,in addition,an aberrant polypeptide with a mol.wt of 21 kDa is also detected as a product of proteolytic cleavage of the mutant precursor molecule(PMID: 9425233).AGA can exsit as a dimer with the mol.wt between 100 kDa and 49 kDa and His124 at an interface between two heterodimers of AGA is crucial for the thermodynamically stable oligomeric structure of AGA.(PMID:9737998). It can also exsit as a heterotetrameric protein of 88 kDa(PMID:1733831).
实验方案
Product Specific Protocols | |
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WB protocol for AGA antibody 83442-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |