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验证数据展示

  • IHC staining of human gliomas using 14690-1-AP

    IHC staining of human gliomas using 14690-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human brain using 14690-1-AP

    WB analysis of human brain using 14690-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14690-1-AP

    IF Staining of HeLa using 14690-1-AP

    Immunofluorescent analysis of Hela cells, using AGAP2 antibody 14690-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse brain using 14690-1-AP

    IP experiment of mouse brain using 14690-1-AP

    IP result of anti-AGAP2 (IP:14690-1-AP, 4ug; Detection:14690-1-AP 1:500) with mouse brain tissue lysate 4000ug.

AGAP2 Polyclonal antibody

AGAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14690-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Cnt-g1, CENTG1, Centaurin-gamma-1, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, AGAP-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14690-1-AP targets AGAP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 AGAP2 fusion protein Ag6142 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
别名 Cnt-g1, CENTG1, Centaurin-gamma-1, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, AGAP-2
计算分子量 125 kDa
观测分子量 124 kDa
GenBank蛋白编号BC028020
基因名称 AGAP2
Gene ID (NCBI) 116986
RRIDAB_2224214
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99490
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AGAP2 antibody 14690-1-APDownload protocol
IHC protocol for AGAP2 antibody 14690-1-APDownload protocol
IF protocol for AGAP2 antibody 14690-1-APDownload protocol
IP protocol for AGAP2 antibody 14690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Phytomedicine

Demethylzeylasteral attenuates hepatic stellate cell activation and liver fibrosis by inhibiting AGAP2 mediated signaling.

Authors - Ke Chen
View Article