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AGER/RAGE Polyclonal antibody

AGER/RAGE Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 32053-1-AP

Print datasheet

Synonyms

RAGE, AGER, Advanced glycosylation end product-specific receptor

验证数据展示

  • WB analysis using 32053-1-AP

    WB analysis using 32053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32053-1-AP (AGER/RAGE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32053-1-AP targets AGER/RAGE in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name advanced glycosylation end product-specific receptor
Synonyms RAGE, AGER, Advanced glycosylation end product-specific receptor
Calculated Molecular Weight43 kDa
Observed Molecular Weight42-55 kDa
Gene Symbol Ager
Gene ID (NCBI) 81722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ63495
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).

实验方案

Product Specific Protocols
WB protocol for AGER/RAGE antibody 32053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols