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AGFG1 Polyclonal antibody

AGFG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12670-1-AP

Print datasheet

Synonyms

AGFG1, ArfGAP with FG repeats 1, HIV 1 Rev binding protein, HRB, Nucleoporin like protein RIP, RAB, Rev interacting protein, RIP

验证数据展示

  • WB analysis of mouse testis using 12670-1-AP

    WB analysis of mouse testis using 12670-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12670-1-AP

    IHC staining of human breast cancer using 12670-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12670-1-AP (AGFG1 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of mouse testis using 12670-1-AP

    IP experiment of mouse testis using 12670-1-AP

    IP result of anti-AGFG1 (IP:12670-1-AP, 4ug; Detection:12670-1-AP 1:400) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse testis tissue, HeLa cells, HepG2 cells, HT-1080 cells, Jurkat cells, K-562 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12670-1-AP targets AGFG1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen AGFG1 fusion protein Ag3352 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ArfGAP with FG repeats 1
Synonyms AGFG1, ArfGAP with FG repeats 1, HIV 1 Rev binding protein, HRB, Nucleoporin like protein RIP, RAB, Rev interacting protein, RIP
Calculated Molecular Weight 562 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC030592
Gene Symbol AGFG1
Gene ID (NCBI) 3267
RRIDAB_2258077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52594
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AGFG1 antibody 12670-1-APDownload protocol
IHC protocol for AGFG1 antibody 12670-1-APDownload protocol
IP protocol for AGFG1 antibody 12670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Cell

A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.

Authors - Peng Dai
View Article

Proc Natl Acad Sci U S A

Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.

Authors - Yoshinori Kanemori
View Article