• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

AGFG2 Polyclonal antibody

AGFG2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11919-1-AP

Print datasheet

Synonyms

RABR, RAB R, HRBL, HIV-1 Rev-binding protein-like protein, ArfGAP with FG repeats 2

验证数据展示

  • WB analysis of Jurkat using 11919-1-AP

    WB analysis of Jurkat using 11919-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11919-1-AP (AGFG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11919-1-AP

    IHC staining of human lymphoma using 11919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11919-1-AP (AGFG2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 11919-1-AP

    IF Staining of HepG2 using 11919-1-AP

    Immunofluorescent analysis of HepG2 cells, using AGFG2 antibody 11919-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11919-1-AP targets AGFG2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AGFG2 fusion protein Ag2546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ArfGAP with FG repeats 2
Synonyms RABR, RAB R, HRBL, HIV-1 Rev-binding protein-like protein, ArfGAP with FG repeats 2
Calculated Molecular Weight 481 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC017329
Gene Symbol AGFG2
Gene ID (NCBI) 3268
RRIDAB_2224646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AGFG2 antibody 11919-1-APDownload protocol
IHC protocol for AGFG2 antibody 11919-1-APDownload protocol
IF protocol for AGFG2 antibody 11919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols