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AGPAT1 Polyclonal antibody

AGPAT1 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10601-1-AP

Print datasheet

Synonyms

1 AGP acyltransferase 1, 1 AGPAT 1, 1 AGPAT1, AGPAT1, G15, LPAAT alpha, LPAATA, Protein G15

验证数据展示

  • IHC staining of human skeletal muscle using 10601-1-AP

    IHC staining of human skeletal muscle using 10601-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10601-1-AP (AGPAT1 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of Raji using 10601-1-AP

    IP experiment of Raji using 10601-1-AP

    IP result of anti-AGPAT1 (IP:10601-1-AP, 4ug; Detection:10601-1-AP 1:300) with Raji cells lysate 2000ug.

经过测试的应用

Positive IP detected inRaji cells, mouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10601-1-AP targets AGPAT1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AGPAT1 fusion protein Ag0837 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha)
Synonyms 1 AGP acyltransferase 1, 1 AGPAT 1, 1 AGPAT1, AGPAT1, G15, LPAAT alpha, LPAATA, Protein G15
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC003007
Gene Symbol AGPAT1
Gene ID (NCBI) 10554
RRIDAB_2224932
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99943
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for AGPAT1 antibody 10601-1-APDownload protocol
IP protocol for AGPAT1 antibody 10601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Metab

A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells

Authors - Shohei Matsufuji
View Article