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AGPAT2 Polyclonal antibody

AGPAT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31483-1-AP

Print datasheet

Synonyms

1 AGP acyltransferase 2, 1 AGPAT 2, 1 AGPAT2, AGPAT2, BSCL, BSCL1, LPAAB, LPAAT beta

验证数据展示

  • WB analysis using 31483-1-AP

    WB analysis using 31483-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31483-1-AP (AGPAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inL02 cells, THP-1 cells, human adipose tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31483-1-AP targets AGPAT2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen AGPAT2 fusion protein Ag34761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
Synonyms 1 AGP acyltransferase 2, 1 AGPAT 2, 1 AGPAT2, AGPAT2, BSCL, BSCL1, LPAAB, LPAAT beta
Calculated Molecular Weight 31 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC019292
Gene Symbol AGPAT2
Gene ID (NCBI) 10555
RRIDAB_3670000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO15120
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2) belongs to a family of enzymes catalyzing the sn-2 acylation of the glycerol-3-phosphate backbone. AGPAT2 is highly expressed in adipose tissues, liver, and skeletal muscle. AGPAT2 is the only AGPAT isoform whose loss-of-function mutations cause a severe form of human congenital generalized lipodystrophy (PMID: 34824276). Human and mouse AGPAT2 have a calculated molecular mass of 31 kDa, and an alternatively spliced form of AGPAT2 mRNA, encoding a protein of 246 rather than 278 amino acids, is also found in human (PMID: 19336658). Western detected AGPAT2 at an apparent molecular mass of 27 kDa.

实验方案

Product Specific Protocols
WB protocol for AGPAT2 antibody 31483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols