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AGPAT2 Polyclonal antibody

AGPAT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31483-1-AP

Print datasheet

Synonyms

1 AGP acyltransferase 2, 1 AGPAT 2, 1 AGPAT2, AGPAT2, BSCL, BSCL1, LPAAB, LPAAT beta

验证数据展示

  • WB analysis using 31483-1-AP

    WB analysis using 31483-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31483-1-AP (AGPAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inL02 cells, THP-1 cells, human adipose tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31483-1-AP targets AGPAT2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen AGPAT2 fusion protein Ag34761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
Synonyms 1 AGP acyltransferase 2, 1 AGPAT 2, 1 AGPAT2, AGPAT2, BSCL, BSCL1, LPAAB, LPAAT beta
Calculated Molecular Weight 31 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC019292
Gene Symbol AGPAT2
Gene ID (NCBI) 10555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO15120
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2) belongs to a family of enzymes catalyzing the sn-2 acylation of the glycerol-3-phosphate backbone. AGPAT2 is highly expressed in adipose tissues, liver, and skeletal muscle. AGPAT2 is the only AGPAT isoform whose loss-of-function mutations cause a severe form of human congenital generalized lipodystrophy (PMID: 34824276). Human and mouse AGPAT2 have a calculated molecular mass of 31 kDa, and an alternatively spliced form of AGPAT2 mRNA, encoding a protein of 246 rather than 278 amino acids, is also found in human (PMID: 19336658). Western detected AGPAT2 at an apparent molecular mass of 27 kDa.

实验方案

Product Specific Protocols
WB protocol for AGPAT2 antibody 31483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols