• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83349-3-RR

    WB analysis using 83349-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83349-3-RR (AGPAT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83349-3-RR

    IHC staining of human ovary cancer using 83349-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83349-3-RR (AGPAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A549 using 83349-3-RR

    FC experiment of A549 using 83349-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug AGPAT2 Recombinant antibody (83349-3-RR, Clone:240153C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83349-3-RR

    Affinity and Kinetic Characterization of 83349-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83349-3-RR against Human AGPAT2 were performed. The affinity constant is 2.72 nM.

AGPAT2 Recombinant antibody

AGPAT2 Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83349-3-RR

产品说明书

CloneNo. 240153C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

240153C2, 1-AGPAT 2, 1-AGP acyltransferase 2, 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, 1-acylglycerol-3-phosphate O-acyltransferase 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83349-3-RR targets AGPAT2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 AGPAT2 fusion protein Ag34761 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
别名 240153C2, 1-AGPAT 2, 1-AGP acyltransferase 2, 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, 1-acylglycerol-3-phosphate O-acyltransferase 2
计算分子量 31 kDa
观测分子量27 kDa
GenBank蛋白编号BC019292
基因名称 AGPAT2
Gene ID (NCBI) 10555
RRIDAB_3671007
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15120
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2) belongs to a family of enzymes catalyzing the sn-2 acylation of the glycerol-3-phosphate backbone. AGPAT2 is highly expressed in adipose tissues, liver, and skeletal muscle. AGPAT2 is the only AGPAT isoform whose loss-of-function mutations cause a severe form of human congenital generalized lipodystrophy (PMID: 34824276). Human and mouse AGPAT2 have a calculated molecular mass of 31 kDa, and an alternatively spliced form of AGPAT2 mRNA, encoding a protein of 246 rather than 278 amino acids, is also found in human (PMID: 19336658). Western detected AGPAT2 at an apparent molecular mass of 27 kDa.

实验方案

Product Specific Protocols
WB protocol for AGPAT2 antibody 83349-3-RRDownload protocol
IHC protocol for AGPAT2 antibody 83349-3-RRDownload protocol
FC protocol for AGPAT2 antibody 83349-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols