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AGPAT2 Recombinant antibody

AGPAT2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240153C2

Cat no : 83349-3-RR

Print datasheet

Synonyms

240153C2, 1-AGPAT 2, 1-AGP acyltransferase 2, 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, 1-acylglycerol-3-phosphate O-acyltransferase 2

验证数据展示

  • WB analysis using 83349-3-RR

    WB analysis using 83349-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83349-3-RR (AGPAT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83349-3-RR

    IHC staining of human ovary cancer using 83349-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83349-3-RR (AGPAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A549 using 83349-3-RR

    FC experiment of A549 using 83349-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug AGPAT2 Recombinant antibody (83349-3-RR, Clone:240153C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83349-3-RR

    Affinity and Kinetic Characterization of 83349-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83349-3-RR against Human AGPAT2 were performed. The affinity constant is 2.72 nM.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83349-3-RR is AGPAT2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen AGPAT2 fusion protein Ag34761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
Synonyms 240153C2, 1-AGPAT 2, 1-AGP acyltransferase 2, 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, 1-acylglycerol-3-phosphate O-acyltransferase 2
Calculated Molecular Weight 31 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC019292
Gene Symbol AGPAT2
Gene ID (NCBI) 10555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15120
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2) belongs to a family of enzymes catalyzing the sn-2 acylation of the glycerol-3-phosphate backbone. AGPAT2 is highly expressed in adipose tissues, liver, and skeletal muscle. AGPAT2 is the only AGPAT isoform whose loss-of-function mutations cause a severe form of human congenital generalized lipodystrophy (PMID: 34824276). Human and mouse AGPAT2 have a calculated molecular mass of 31 kDa, and an alternatively spliced form of AGPAT2 mRNA, encoding a protein of 246 rather than 278 amino acids, is also found in human (PMID: 19336658). Western detected AGPAT2 at an apparent molecular mass of 27 kDa.

实验方案

Product Specific Protocols
WB protocol for AGPAT2 antibody 83349-3-RRDownload protocol
IHC protocol for AGPAT2 antibody 83349-3-RRDownload protocol
FC protocol for AGPAT2 antibody 83349-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols