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AGT Monoclonal antibody, PBS Only

AGT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2F5

Cat no : 68020-1-PBS

Print datasheet

Synonyms

AGT, Ang I Angiotensin 2, Ang II Angiotensin 3, Ang III, Angiotensin I, Angiotensin II, Angiotensin III, Angiotensinogen, ANHU, Des Asp[1] angiotensin II, FLJ92595, FLJ97926, Serpin A8, SERPINA8

验证数据展示

  • WB analysis of human plasma using 68020-1-Ig (same clone as 68020-1-PBS)

    WB analysis of human plasma using 68020-1-Ig (same clone as 68020-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

    IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGT antibody included
  • IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

    IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGT antibody included
  • IF Staining of HepG2 using 68020-1-Ig (same clone as 68020-1-PBS)

    IF Staining of HepG2 using 68020-1-Ig (same clone as 68020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AGT antibody (68020-1-Ig, Clone: 1B2F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68020-1-Ig (same clone as 68020-1-PBS)

    WB analysis of human placenta using 68020-1-Ig (same clone as 68020-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AGT Ready-To-Use IHC Kit. AGT primary antibody included.

产品信息

68020-1-PBS targets AGT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, pig
Immunogen AGT fusion protein Ag16658 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Synonyms AGT, Ang I Angiotensin 2, Ang II Angiotensin 3, Ang III, Angiotensin I, Angiotensin II, Angiotensin III, Angiotensinogen, ANHU, Des Asp[1] angiotensin II, FLJ92595, FLJ97926, Serpin A8, SERPINA8
Calculated Molecular Weight 485 aa, 53 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC011519
Gene Symbol AGT
Gene ID (NCBI) 183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01019
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.