验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, A549 cells, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human heart tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14067-1-AP targets CCP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CCP1 fusion protein Ag5122 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP/GTP binding protein 1 |
| 别名 | AGTPBP1, KIAA1035, EC:3.4.17.-, Cytosolic carboxypeptidase 1, ATP/GTP-binding protein 1 |
| 计算分子量 | 138 kDa |
| 观测分子量 | 138 kDa |
| GenBank蛋白编号 | BC060815 |
| 基因名称 | CCP1 |
| Gene ID (NCBI) | 23287 |
| RRID | AB_2225559 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UPW5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AGTPBP1, also known as CCP1 (cytosolic carboxy-peptidase 1), or Nna1, belongs to the peptidase M14 family. AGTPBP1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motiff. Thus, it may contribute to nuclear signaling events in differentiating and regenerating neurons. It serves important functions in the nervous system, and AGTPBP1 mutations may cause Purkinje cell degeneration (pcd) phenotypes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCP1 antibody 14067-1-AP | Download protocol |
| IHC protocol for CCP1 antibody 14067-1-AP | Download protocol |
| IF protocol for CCP1 antibody 14067-1-AP | Download protocol |
| IP protocol for CCP1 antibody 14067-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.
| ||
Nat Commun AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation. | ||
Proc Natl Acad Sci U S A Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy.
| ||
J Cell Biol CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice. |
