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AGXT Polyclonal antibody

AGXT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22394-1-AP

Print datasheet

Synonyms

AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6

验证数据展示

  • WB analysis of mouse liver using 22394-1-AP

    WB analysis of mouse liver using 22394-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22394-1-AP (AGXT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 22394-1-AP

    IHC staining of human liver using 22394-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 22394-1-AP (AGXT Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with AGXT antibody included
  • IHC staining of human liver using 22394-1-AP

    IHC staining of human liver using 22394-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 22394-1-AP (AGXT Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with AGXT antibody included

经过测试的应用

Positive WB detected inmouse liver tissue, mouse lung tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AGXT Ready-To-Use IHC Kit. AGXT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

22394-1-AP targets AGXT in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen AGXT fusion protein Ag18062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alanine-glyoxylate aminotransferase
Synonyms AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6
Calculated Molecular Weight 392 aa, 43 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC132819
Gene Symbol AGXT
Gene ID (NCBI) 189
RRIDAB_2879098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.

实验方案

Product Specific Protocols
WB protocol for AGXT antibody 22394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nucleic Acid Ther

Systemic Alanine Glyoxylate Aminotransferase Messenger RNA Improves Glyoxylate Metabolism in a Mouse Model of Primary Hyperoxaluria Type 1.

Authors - Anjli Kukreja
View Article