验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, mouse lung tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
22394-1-AP targets AGXT in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | IF |
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Immunogen | AGXT fusion protein Ag18062 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | alanine-glyoxylate aminotransferase |
Synonyms | EC:2.6.1.51, EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1 |
Calculated Molecular Weight | 392 aa, 43 kDa |
Observed Molecular Weight | 43-45 kDa |
GenBank Accession Number | BC132819 |
Gene Symbol | AGXT |
Gene ID (NCBI) | 189 |
RRID | AB_2879098 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P21549 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease that is characterized by deficient AGXT, and excessive hepatic oxalate production that leads to renal failure.
实验方案
Product Specific Protocols | |
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WB protocol for AGXT antibody 22394-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |