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AGXT Monoclonal antibody

AGXT Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E12H1

Cat no : 67402-1-Ig

Print datasheet

Synonyms

AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6

验证数据展示

  • WB analysis using 67402-1-Ig

    WB analysis using 67402-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67402-1-Ig

    IHC staining of human liver cancer using 67402-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AGXT antibody included
  • IHC staining of human kidney using 67402-1-Ig

    IHC staining of human kidney using 67402-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AGXT antibody included

经过测试的应用

Positive WB detected inHepG2 cells, NCI-H1299 cells, A549 cells, L02 cells, HuH-7 cells, SMMC-7721 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AGXT Ready-To-Use IHC Kit. AGXT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67402-1-Ig targets AGXT in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen AGXT fusion protein Ag19188 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alanine-glyoxylate aminotransferase
Synonyms AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6
Calculated Molecular Weight 392 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC132819
Gene Symbol AGXT
Gene ID (NCBI) 189
RRIDAB_2882644
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.

实验方案

Product Specific Protocols
WB protocol for AGXT antibody 67402-1-IgDownload protocol
IHC protocol for AGXT antibody 67402-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols