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AGXT2 Monoclonal antibody

AGXT2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3A5B12

Cat no : 66602-1-Ig

Print datasheet

Synonyms

AGT 2, AGT2, AGXT2, Beta ALAAT II, D AIBAT

验证数据展示

  • WB analysis using 66602-1-Ig

    WB analysis using 66602-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66602-1-Ig (AGXT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66602-1-Ig

    IHC staining of human kidney using 66602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66602-1-Ig (AGXT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66602-1-Ig

    IHC staining of human kidney using 66602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66602-1-Ig (AGXT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66602-1-Ig targets AGXT2 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Immunogen AGXT2 fusion protein Ag26888 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alanine-glyoxylate aminotransferase 2
Synonyms AGT 2, AGT2, AGXT2, Beta ALAAT II, D AIBAT
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberNM_001306173
Gene Symbol AGXT2
Gene ID (NCBI) 64902
RRIDAB_2881962
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AGXT2 antibody 66602-1-IgDownload protocol
IHC protocol for AGXT2 antibody 66602-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,CoIP

J Hepatocell Carcinoma

AGXT2 Suppresses the Proliferation and Dissemination of Hepatocellular Carcinoma Cells by Modulating Intracellular Lipid Metabolism

Authors - Tian Chen
  • KD Validated
View Article