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AGXT2L1 Polyclonal antibody

AGXT2L1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17042-1-AP

Print datasheet

Synonyms

AGXT2L1, ETNPPL

验证数据展示

  • WB analysis using 17042-1-AP

    WB analysis using 17042-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17042-1-AP (AGXT2L1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 17042-1-AP

    IHC staining of mouse liver using 17042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17042-1-AP (AGXT2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17042-1-AP

    IHC staining of mouse brain using 17042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17042-1-AP (AGXT2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17042-1-AP targets AGXT2L1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen AGXT2L1 fusion protein Ag10584 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alanine-glyoxylate aminotransferase 2-like 1
Synonyms AGXT2L1, ETNPPL
Calculated Molecular Weight 499 aa, 56 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC022526
Gene Symbol AGXT2L1
Gene ID (NCBI) 64850
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TBG4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), also called ethanolamine phosphate phospholyase (ETNPPL), is a brain enzyme, so-named after its similarity to AGXT2 (36% sequence identity), that may be involved in the pathogenesis of severe neuropsychiatric disturbances(PMID: 23761375). AGXT2L1 is significantly down-regulated in cancerous tissues and may be a tumor suppressor(PMID: 26294768, 32724374).

实验方案

Product Specific Protocols
WB protocol for AGXT2L1 antibody 17042-1-APDownload protocol
IHC protocol for AGXT2L1 antibody 17042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Transl Med

Multiomic analysis of monocyte-derived alveolar macrophages in idiopathic pulmonary fibrosis

Authors - Miaomiao Zhang
View Article