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验证数据展示

  • IHC staining of mouse liver using 83984-5-RR

    IHC staining of mouse liver using 83984-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83984-5-RR (AGXT2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 83984-5-RR

    IF Staining of U2OS using 83984-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using AGXT2L1 antibody (83984-5-RR, Clone: 241119H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83984-5-RR

    Affinity and Kinetic Characterization of 83984-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83984-5-RR against Human AGXT2L1 were performed. The affinity constant is 1.77 nM.

AGXT2L1 Recombinant antibody

AGXT2L1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83984-5-RR

产品说明书

CloneNo. 241119H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

ETNPPL, Ethanolamine-phosphate phospho-lyase, EC:4.2.3.2, Alanine--glyoxylate aminotransferase 2-like 1, 241119H5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83984-5-RR targets AGXT2L1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 AGXT2L1 fusion protein Ag10584 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alanine-glyoxylate aminotransferase 2-like 1
别名 ETNPPL, Ethanolamine-phosphate phospho-lyase, EC:4.2.3.2, Alanine--glyoxylate aminotransferase 2-like 1, 241119H5
计算分子量 499 aa, 56 kDa
GenBank蛋白编号BC022526
基因名称 AGXT2L1
Gene ID (NCBI) 64850
RRIDAB_3671558
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TBG4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), also called ethanolamine phosphate phospholyase (ETNPPL), is a brain enzyme, so-named after its similarity to AGXT2 (36% sequence identity), that may be involved in the pathogenesis of severe neuropsychiatric disturbances(PMID: 23761375). AGXT2L1 is significantly down-regulated in cancerous tissues and may be a tumor suppressor(PMID: 26294768,

实验方案

Product Specific Protocols
IHC protocol for AGXT2L1 antibody 83984-5-RRDownload protocol
IF protocol for AGXT2L1 antibody 83984-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols