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AGXT2L2 Polyclonal antibody

AGXT2L2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16449-1-AP

Print datasheet

Synonyms

5-phosphohydroxy-L-lysine phospho-lyase, AGXT2L2, PHYKPL

验证数据展示

  • WB analysis using 16449-1-AP

    WB analysis using 16449-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16449-1-AP (AGXT2L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 16449-1-AP

    IHC staining of human prostate cancer using 16449-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16449-1-AP (AGXT2L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 16449-1-AP

    IHC staining of human prostate cancer using 16449-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16449-1-AP (AGXT2L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, rat heart
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16449-1-AP targets AGXT2L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AGXT2L2 fusion protein Ag8391 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alanine-glyoxylate aminotransferase 2-like 2
Synonyms 5-phosphohydroxy-L-lysine phospho-lyase, AGXT2L2, PHYKPL
Calculated Molecular Weight450aa,50 kDa; 175aa,19 kDa
GenBank Accession NumberBC008009
Gene Symbol AGXT2L2
Gene ID (NCBI) 85007
RRIDAB_2225868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUZ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AGXT2L2 antibody 16449-1-APDownload protocol
IHC protocol for AGXT2L2 antibody 16449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols