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  • KD/KO Validated

AHCY Polyclonal antibody

AHCY Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10757-2-AP

Print datasheet

Synonyms

SAHH, EC:3.13.2.1



经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells, mouse liver tissue, HeLa cells, Jurkat cells, rat liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AHCY Ready-To-Use IHC Kit. AHCY primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10757-2-AP targets AHCY in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, branchiostoma belcheri
Immunogen AHCY fusion protein Ag1208 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S-adenosylhomocysteine hydrolase
Synonyms SAHH, EC:3.13.2.1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC011606
Gene Symbol AHCY
Gene ID (NCBI) 191
RRIDAB_2289488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

实验方案

Product Specific Protocols
WB protocol for AHCY antibody 10757-2-APDownload protocol
IHC protocol for AHCY antibody 10757-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh
humanWB, IP

Nat Commun

H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.

Authors - Jichun Zhou
  • KD Validated
humanWB

Autophagy

AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner.

Authors - Wei Huang
  • KD Validated
humanWB

Oncogene

PMID: 27775072

Authors - T Zhong
humanWB,IHC

Clin Cancer Res

Digital transcript profile analysis with aRNA-LongSAGE validates FERMT1 as a potential novel prognostic marker for colon cancer.

Authors - Fan Junwei J
mouseWB

Arterioscler Thromb Vasc Biol

Increased plasma S-adenosylhomocysteine-accelerated atherosclerosis is associated with epigenetic regulation of endoplasmic reticulum stress in apoE-/- mice.

Authors - Yunjun Xiao
  • KD Validated