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AHCY Monoclonal antibody, PBS Only

AHCY Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9B10

Cat no : 66019-1-PBS

Print datasheet

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

验证数据展示

  • WB analysis using 66019-1-Ig (same clone as 66019-1-PBS)

    WB analysis using 66019-1-Ig (same clone as 66019-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)

    IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHCY antibody included
  • IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)

    IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHCY antibody included
  • IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AHCY antibody (66019-1-Ig, Clone: 1F9B10 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AHCY Ready-To-Use IHC Kit. AHCY primary antibody included.

产品信息

66019-1-PBS targets AHCY in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen AHCY fusion protein Ag18036 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name S-adenosylhomocysteine hydrolase
Synonyms Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC011606
Gene Symbol AHCY
Gene ID (NCBI) 191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23526
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).