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验证数据展示

  • WB analysis using 83369-5-RR

    WB analysis using 83369-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83369-5-RR (AHR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83369-5-RR

    IHC staining of human ovary cancer using 83369-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83369-5-RR (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AHR antibody included

AHR Recombinant antibody

AHR Recombinant Antibody for WB, IHC, ELISA
Cat No. 83369-5-RR

Print datasheet

CloneNo. 240325G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 240325G8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83369-5-RR targets AHR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen AHR fusion protein Ag12193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name aryl hydrocarbon receptor
Synonyms Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 240325G8
Calculated Molecular Weight 848 aa, 96 kDa
Observed Molecular Weight105-110 kDa
GenBank Accession NumberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
RRIDAB_3671022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP35869
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

实验方案

Product Specific Protocols
WB protocol for AHR antibody 83369-5-RRDownload protocol
IHC protocol for AHR antibody 83369-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols