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Fetuin-A/AHSG Monoclonal antibody

Fetuin-A/AHSG Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1F6B9

Cat no : 66094-1-Ig

Print datasheet

Synonyms

AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS

验证数据展示

  • WB analysis of HepG2 using 66094-1-Ig

    WB analysis of HepG2 using 66094-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 66094-1-Ig

    IP experiment of HepG2 using 66094-1-Ig

    IP result of anti-Fetuin-A (IP:66094-1-Ig, 3ug; Detection:66094-1-Ig 1:3000) with HepG2 cells lysate 6000ug.

  • IHC staining of human liver cancer using 66094-1-Ig

    IHC staining of human liver cancer using 66094-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with AHSG/Fetuin A antibody included
  • IHC staining of human liver cancer using 66094-1-Ig

    IHC staining of human liver cancer using 66094-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with AHSG/Fetuin A antibody included
  • IF Staining of HepG2 using 66094-1-Ig

    IF Staining of HepG2 using 66094-1-Ig

    Immunofluorescent analysis of HepG2 cells, using AHSG antibody 66094-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

  • FC experiment of HepG2 using 66094-1-Ig

    FC experiment of HepG2 using 66094-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig, Clone:1F6B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepg2 cells, human heart tissue, human plasma tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AHSG/Fetuin A Ready-To-Use IHC Kit. AHSG/Fetuin A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66094-1-Ig targets Fetuin-A/AHSG in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Fetuin-A/AHSG fusion protein Ag10073 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name alpha-2-HS-glycoprotein
Synonyms AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS
Calculated Molecular Weight 367 aa, 39 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC048198
Gene Symbol Fetuin-A
Gene ID (NCBI) 197
RRIDAB_11232222
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02765
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.

实验方案

Product Specific Protocols
WB protocol for Fetuin-A/AHSG antibody 66094-1-IgDownload protocol
IHC protocol for Fetuin-A/AHSG antibody 66094-1-IgDownload protocol
IF protocol for Fetuin-A/AHSG antibody 66094-1-IgDownload protocol
IP protocol for Fetuin-A/AHSG antibody 66094-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

Fetal hepatocytes protect the HSPC genome via fetuin-A

Authors - Xiao-Lin Guo
View Article