验证数据展示
经过测试的应用
| Positive WB detected in | Hepg2 cells, human heart tissue, human plasma tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
66094-1-Ig targets Fetuin-A/AHSG in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Fetuin-A/AHSG fusion protein Ag10073 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | alpha-2-HS-glycoprotein |
| Synonyms | AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS |
| Calculated Molecular Weight | 367 aa, 39 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC048198 |
| Gene Symbol | Fetuin-A |
| Gene ID (NCBI) | 197 |
| RRID | AB_11232222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02765 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Fetuin-A/AHSG antibody 66094-1-Ig | Download protocol |
| IHC protocol for Fetuin-A/AHSG antibody 66094-1-Ig | Download protocol |
| IF protocol for Fetuin-A/AHSG antibody 66094-1-Ig | Download protocol |
| IP protocol for Fetuin-A/AHSG antibody 66094-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
